A Combined Approach of High-Throughput Sequencing and Degradome Analysis Reveals Tissue Specific Expression of MicroRNAs and Their Targets in Cucumber
Identifieur interne : 001432 ( Main/Exploration ); précédent : 001431; suivant : 001433A Combined Approach of High-Throughput Sequencing and Degradome Analysis Reveals Tissue Specific Expression of MicroRNAs and Their Targets in Cucumber
Auteurs : Weihua Mao [République populaire de Chine] ; Zeyun Li [République populaire de Chine] ; Xiaojian Xia [République populaire de Chine] ; Yadan Li [République populaire de Chine] ; Jingquan Yu [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
MicroRNAs (miRNAs) are endogenous small RNAs playing an important regulatory function in plant development and stress responses. Among them, some are evolutionally conserved in plant and others are only expressed in certain species, tissue or developmental stages. Cucumber is among the most important greenhouse species in the world, but only a limited number of miRNAs from cucumber have been identified and the experimental validation of the related miRNA targets is still lacking. In this study, two independent small RNA libraries from cucumber leaves and roots were constructed, respectively, and sequenced with the high-throughput Illumina Solexa system. Based on sequence similarity and hairpin structure prediction, a total of 29 known miRNA families and 2 novel miRNA families containing a total of 64 miRNA were identified. QRT-PCR analysis revealed that some of the cucumber miRNAs were preferentially expressed in certain tissues. With the recently developed ‘high throughput degradome sequencing’ approach, 21 target mRNAs of known miRNAs were identified for the first time in cucumber. These targets were associated with development, reactive oxygen species scavenging, signaling transduction and transcriptional regulation. Our study provides an overview of miRNA expression profile and interaction between miRNA and target, which will help further understanding of the important roles of miRNAs in cucumber plants.
Url:
DOI: 10.1371/journal.pone.0033040
PubMed: 22479356
PubMed Central: 3316546
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 001106
- to stream Pmc, to step Curation: 001105
- to stream Pmc, to step Checkpoint: 000D83
- to stream Ncbi, to step Merge: 000F58
- to stream Ncbi, to step Curation: 000F58
- to stream Ncbi, to step Checkpoint: 000F58
- to stream Main, to step Merge: 001447
- to stream Main, to step Curation: 001432
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Combined Approach of High-Throughput Sequencing and Degradome Analysis Reveals Tissue Specific Expression of MicroRNAs and Their Targets in Cucumber</title><author><name sortKey="Mao, Weihua" sort="Mao, Weihua" uniqKey="Mao W" first="Weihua" last="Mao">Weihua Mao</name><affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Department of Horticulture, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Horticulture, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Center of Analysis and Measurement, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Analysis and Measurement, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author><author><name sortKey="Li, Zeyun" sort="Li, Zeyun" uniqKey="Li Z" first="Zeyun" last="Li">Zeyun Li</name><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Center of Analysis and Measurement, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Analysis and Measurement, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author><author><name sortKey="Xia, Xiaojian" sort="Xia, Xiaojian" uniqKey="Xia X" first="Xiaojian" last="Xia">Xiaojian Xia</name><affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Department of Horticulture, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Horticulture, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author><author><name sortKey="Li, Yadan" sort="Li, Yadan" uniqKey="Li Y" first="Yadan" last="Li">Yadan Li</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Hunan Agricultural Bioengineering Research Institute, Hunan Agricultural University, Changsha, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hunan Agricultural Bioengineering Research Institute, Hunan Agricultural University, Changsha</wicri:regionArea><wicri:noRegion>Changsha</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Jingquan" sort="Yu, Jingquan" uniqKey="Yu J" first="Jingquan" last="Yu">Jingquan Yu</name><affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Department of Horticulture, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Horticulture, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22479356</idno><idno type="pmc">3316546</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316546</idno><idno type="RBID">PMC:3316546</idno><idno type="doi">10.1371/journal.pone.0033040</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">001106</idno><idno type="wicri:Area/Pmc/Curation">001105</idno><idno type="wicri:Area/Pmc/Checkpoint">000D83</idno><idno type="wicri:Area/Ncbi/Merge">000F58</idno><idno type="wicri:Area/Ncbi/Curation">000F58</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F58</idno><idno type="wicri:Area/Main/Merge">001447</idno><idno type="wicri:Area/Main/Curation">001432</idno><idno type="wicri:Area/Main/Exploration">001432</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Combined Approach of High-Throughput Sequencing and Degradome Analysis Reveals Tissue Specific Expression of MicroRNAs and Their Targets in Cucumber</title><author><name sortKey="Mao, Weihua" sort="Mao, Weihua" uniqKey="Mao W" first="Weihua" last="Mao">Weihua Mao</name><affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Department of Horticulture, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Horticulture, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Center of Analysis and Measurement, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Analysis and Measurement, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author><author><name sortKey="Li, Zeyun" sort="Li, Zeyun" uniqKey="Li Z" first="Zeyun" last="Li">Zeyun Li</name><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Center of Analysis and Measurement, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Analysis and Measurement, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author><author><name sortKey="Xia, Xiaojian" sort="Xia, Xiaojian" uniqKey="Xia X" first="Xiaojian" last="Xia">Xiaojian Xia</name><affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Department of Horticulture, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Horticulture, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author><author><name sortKey="Li, Yadan" sort="Li, Yadan" uniqKey="Li Y" first="Yadan" last="Li">Yadan Li</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Hunan Agricultural Bioengineering Research Institute, Hunan Agricultural University, Changsha, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hunan Agricultural Bioengineering Research Institute, Hunan Agricultural University, Changsha</wicri:regionArea><wicri:noRegion>Changsha</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Jingquan" sort="Yu, Jingquan" uniqKey="Yu J" first="Jingquan" last="Yu">Jingquan Yu</name><affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Department of Horticulture, Zhejiang University, Hangzhou, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Horticulture, Zhejiang University, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>MicroRNAs (miRNAs) are endogenous small RNAs playing an important regulatory function in plant development and stress responses. Among them, some are evolutionally conserved in plant and others are only expressed in certain species, tissue or developmental stages. Cucumber is among the most important greenhouse species in the world, but only a limited number of miRNAs from cucumber have been identified and the experimental validation of the related miRNA targets is still lacking. In this study, two independent small RNA libraries from cucumber leaves and roots were constructed, respectively, and sequenced with the high-throughput Illumina Solexa system. Based on sequence similarity and hairpin structure prediction, a total of 29 known miRNA families and 2 novel miRNA families containing a total of 64 miRNA were identified. QRT-PCR analysis revealed that some of the cucumber miRNAs were preferentially expressed in certain tissues. With the recently developed ‘high throughput degradome sequencing’ approach, 21 target mRNAs of known miRNAs were identified for the first time in cucumber. These targets were associated with development, reactive oxygen species scavenging, signaling transduction and transcriptional regulation. Our study provides an overview of miRNA expression profile and interaction between miRNA and target, which will help further understanding of the important roles of miRNAs in cucumber plants.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Llave, C" uniqKey="Llave C">C Llave</name></author><author><name sortKey="Kasschau, Kd" uniqKey="Kasschau K">KD Kasschau</name></author><author><name sortKey="Rector, Ma" uniqKey="Rector M">MA Rector</name></author><author><name sortKey="Carrington, Jc" uniqKey="Carrington J">JC Carrington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author><author><name sortKey="Chinnusamy, V" uniqKey="Chinnusamy V">V Chinnusamy</name></author><author><name sortKey="Zhu, Jh" uniqKey="Zhu J">JH Zhu</name></author><author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shukla, Li" uniqKey="Shukla L">LI Shukla</name></author><author><name sortKey="Chinnusamy, V" uniqKey="Chinnusamy V">V Chinnusamy</name></author><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chuck, G" uniqKey="Chuck G">G Chuck</name></author><author><name sortKey="Candela, H" uniqKey="Candela H">H Candela</name></author><author><name sortKey="Hake, S" uniqKey="Hake S">S Hake</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bonnet, E" uniqKey="Bonnet E">E Bonnet</name></author><author><name sortKey="Wuyts, J" uniqKey="Wuyts J">J Wuyts</name></author><author><name sortKey="Rouze, P" uniqKey="Rouze P">P Rouze</name></author><author><name sortKey="Van De Peer, Y" uniqKey="Van De Peer Y">Y Van de Peer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adai, A" uniqKey="Adai A">A Adai</name></author><author><name sortKey="Johnson, C" uniqKey="Johnson C">C Johnson</name></author><author><name sortKey="Mlotshwa, S" uniqKey="Mlotshwa S">S Mlotshwa</name></author><author><name sortKey="Archer Evans, S" uniqKey="Archer Evans S">S Archer-evans</name></author><author><name sortKey="Manocha, V" uniqKey="Manocha V">V Manocha</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williams, L" uniqKey="Williams L">L Williams</name></author><author><name sortKey="Carles, Cc" uniqKey="Carles C">CC Carles</name></author><author><name sortKey="Osmont, Ks" uniqKey="Osmont K">KS Osmont</name></author><author><name sortKey="Fletcher, Jc" uniqKey="Fletcher J">JC Fletcher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Bh" uniqKey="Zhang B">BH Zhang</name></author><author><name sortKey="Pan, Xp" uniqKey="Pan X">XP Pan</name></author><author><name sortKey="Wang, Ql" uniqKey="Wang Q">QL Wang</name></author><author><name sortKey="Cobb, Gp" uniqKey="Cobb G">GP Cobb</name></author><author><name sortKey="Aanderson, Ta" uniqKey="Aanderson T">TA Aanderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pilcher, Rlr" uniqKey="Pilcher R">RLR Pilcher</name></author><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Pakseresht, N" uniqKey="Pakseresht N">N Pakseresht</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author><author><name sortKey="Manning, K" uniqKey="Manning K">K Manning</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, C" uniqKey="Lu C">C Lu</name></author><author><name sortKey="Tej, Ss" uniqKey="Tej S">SS Tej</name></author><author><name sortKey="Luo, Sj" uniqKey="Luo S">SJ Luo</name></author><author><name sortKey="Haudenschild, Cd" uniqKey="Haudenschild C">CD Haudenschild</name></author><author><name sortKey="Meyers, Bc" uniqKey="Meyers B">BC Meyers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rajagopalan, R" uniqKey="Rajagopalan R">R Rajagopalan</name></author><author><name sortKey="Vaucheret, H" uniqKey="Vaucheret H">H Vaucheret</name></author><author><name sortKey="Trejo, J" uniqKey="Trejo J">J Trejo</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fahlgren, N" uniqKey="Fahlgren N">N Fahlgren</name></author><author><name sortKey="Howell, Md" uniqKey="Howell M">MD Howell</name></author><author><name sortKey="Kasschau, Kd" uniqKey="Kasschau K">KD Kasschau</name></author><author><name sortKey="Chapman, Ej" uniqKey="Chapman E">EJ Chapman</name></author><author><name sortKey="Sullivan, Cm" uniqKey="Sullivan C">CM Sullivan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Subramanian, S" uniqKey="Subramanian S">S Subramanian</name></author><author><name sortKey="Fu, Y" uniqKey="Fu Y">Y Fu</name></author><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author><author><name sortKey="Barbazuk, Wb" uniqKey="Barbazuk W">WB Barbazuk</name></author><author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author><author><name sortKey="Zhou, Xf" uniqKey="Zhou X">XF Zhou</name></author><author><name sortKey="Zheng, Y" uniqKey="Zheng Y">Y Zheng</name></author><author><name sortKey="Zhang, Wx" uniqKey="Zhang W">WX Zhang</name></author><author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arenas Huertero, C" uniqKey="Arenas Huertero C">C Arenas-Huertero</name></author><author><name sortKey="Perez, B" uniqKey="Perez B">B Perez</name></author><author><name sortKey="Rabanal, F" uniqKey="Rabanal F">F Rabanal</name></author><author><name sortKey="Blanco Melo, D" uniqKey="Blanco Melo D">D Blanco-Melo</name></author><author><name sortKey="De La Rosa, C" uniqKey="De La Rosa C">C De la Rosa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lelandais Briere, C" uniqKey="Lelandais Briere C">C Lelandais-Briere</name></author><author><name sortKey="Naya, L" uniqKey="Naya L">L Naya</name></author><author><name sortKey="Sallet, E" uniqKey="Sallet E">E Sallet</name></author><author><name sortKey="Calenge, F" uniqKey="Calenge F">F Calenge</name></author><author><name sortKey="Frugier, F" uniqKey="Frugier F">F Frugier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pantaleo, V" uniqKey="Pantaleo V">V Pantaleo</name></author><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Miozzi, L" uniqKey="Miozzi L">L Miozzi</name></author><author><name sortKey="Moulton, V" uniqKey="Moulton V">V Moulton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rhoades, Mw" uniqKey="Rhoades M">MW Rhoades</name></author><author><name sortKey="Reinhart, Bj" uniqKey="Reinhart B">BJ Reinhart</name></author><author><name sortKey="Lim, Lp" uniqKey="Lim L">LP Lim</name></author><author><name sortKey="Burge, Cb" uniqKey="Burge C">CB Burge</name></author><author><name sortKey="Bartel, B" uniqKey="Bartel B">B Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chaudhuri, K" uniqKey="Chaudhuri K">K Chaudhuri</name></author><author><name sortKey="Chatterjee, R" uniqKey="Chatterjee R">R Chatterjee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jones Rhoades, Mw" uniqKey="Jones Rhoades M">MW Jones-Rhoades</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jones Rhoades, Mw" uniqKey="Jones Rhoades M">MW Jones-Rhoades</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author><author><name sortKey="Bartel, B" uniqKey="Bartel B">B Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Addo Quaye, C" uniqKey="Addo Quaye C">C Addo-Quaye</name></author><author><name sortKey="Eshoo, Tw" uniqKey="Eshoo T">TW Eshoo</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="German, Ma" uniqKey="German M">MA German</name></author><author><name sortKey="Pillay, M" uniqKey="Pillay M">M Pillay</name></author><author><name sortKey="Jeong, Dh" uniqKey="Jeong D">DH Jeong</name></author><author><name sortKey="Hetawal, A" uniqKey="Hetawal A">A Hetawal</name></author><author><name sortKey="Luo, Sj" uniqKey="Luo S">SJ Luo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Yf" uniqKey="Li Y">YF Li</name></author><author><name sortKey="Zheng, Y" uniqKey="Zheng Y">Y Zheng</name></author><author><name sortKey="Addo Quaye, C" uniqKey="Addo Quaye C">C Addo-Quaye</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name></author><author><name sortKey="Saini, A" uniqKey="Saini A">A Saini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, Qx" uniqKey="Song Q">QX Song</name></author><author><name sortKey="Liu, Yf" uniqKey="Liu Y">YF Liu</name></author><author><name sortKey="Hu, Xy" uniqKey="Hu X">XY Hu</name></author><author><name sortKey="Zhang, Wk" uniqKey="Zhang W">WK Zhang</name></author><author><name sortKey="Ma, Ba" uniqKey="Ma B">BA Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez, G" uniqKey="Martinez G">G Martínez</name></author><author><name sortKey="Forment, J" uniqKey="Forment J">J Forment</name></author><author><name sortKey="Llave, C" uniqKey="Llave C">C Llave</name></author><author><name sortKey="Pallas, V" uniqKey="Pallas V">V Pallás</name></author><author><name sortKey="G Mez, G" uniqKey="G Mez G">G Gómez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morin, Rd" uniqKey="Morin R">RD Morin</name></author><author><name sortKey="Aksay, G" uniqKey="Aksay G">G Aksay</name></author><author><name sortKey="Dolgosheina, E" uniqKey="Dolgosheina E">E Dolgosheina</name></author><author><name sortKey="Ebhardt, Ha" uniqKey="Ebhardt H">HA Ebhardt</name></author><author><name sortKey="Magrini, V" uniqKey="Magrini V">V Magrini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Szittya, G" uniqKey="Szittya G">G Szittya</name></author><author><name sortKey="Moxon, S" uniqKey="Moxon S">S Moxon</name></author><author><name sortKey="Santos, Dm" uniqKey="Santos D">DM Santos</name></author><author><name sortKey="Jing, R" uniqKey="Jing R">R Jing</name></author><author><name sortKey="Fevereiro, Mps" uniqKey="Fevereiro M">MPS Fevereiro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author><author><name sortKey="Wang, Mb" uniqKey="Wang M">MB Wang</name></author><author><name sortKey="Tu, Jx" uniqKey="Tu J">JX Tu</name></author><author><name sortKey="Helliwell, Ca" uniqKey="Helliwell C">CA Helliwell</name></author><author><name sortKey="Waterhouse, Pm" uniqKey="Waterhouse P">PM Waterhouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Bh" uniqKey="Zhang B">BH Zhang</name></author><author><name sortKey="Pan, Xp" uniqKey="Pan X">XP Pan</name></author><author><name sortKey="Cox, Sb" uniqKey="Cox S">SB Cox</name></author><author><name sortKey="Cobb, Gp" uniqKey="Cobb G">GP Cobb</name></author><author><name sortKey="Anderson, Ta" uniqKey="Anderson T">TA Anderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyers, Bc" uniqKey="Meyers B">BC Meyers</name></author><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author><author><name sortKey="Bartel, B" uniqKey="Bartel B">B Bartel</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author><author><name sortKey="Baulcombe, D" uniqKey="Baulcombe D">D Baulcombe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Addo Quaye, C" uniqKey="Addo Quaye C">C Addo-Quaye</name></author><author><name sortKey="Miller, W" uniqKey="Miller W">W Miller</name></author><author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Unver, T" uniqKey="Unver T">T Unver</name></author><author><name sortKey="Budak, H" uniqKey="Budak H">H Budak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xie, Fl" uniqKey="Xie F">FL Xie</name></author><author><name sortKey="Frazier, Tp" uniqKey="Frazier T">TP Frazier</name></author><author><name sortKey="Zhang, Bh" uniqKey="Zhang B">BH Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ashburner, M" uniqKey="Ashburner M">M Ashburner</name></author><author><name sortKey="Bergman, Cm" uniqKey="Bergman C">CM Bergman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, Sw" uniqKey="Huang S">SW Huang</name></author><author><name sortKey="Li, Rg" uniqKey="Li R">RG Li</name></author><author><name sortKey="Zhang, Zh" uniqKey="Zhang Z">ZH Zhang</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Gu, Xf" uniqKey="Gu X">XF Gu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author><author><name sortKey="Jagadeeswaran, G" uniqKey="Jagadeeswaran G">G Jagadeeswaran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author><author><name sortKey="Girke, T" uniqKey="Girke T">T Girke</name></author><author><name sortKey="Jain, Pk" uniqKey="Jain P">PK Jain</name></author><author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Wz" uniqKey="Yang W">WZ Yang</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name></author><author><name sortKey="Zhang, Jg" uniqKey="Zhang J">JG Zhang</name></author><author><name sortKey="Feng, Jl" uniqKey="Feng J">JL Feng</name></author><author><name sortKey="Li, C" uniqKey="Li C">C Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, Hs" uniqKey="Guo H">HS Guo</name></author><author><name sortKey="Xie, Q" uniqKey="Xie Q">Q Xie</name></author><author><name sortKey="Fei, Jf" uniqKey="Fei J">JF Fei</name></author><author><name sortKey="Chua, Nh" uniqKey="Chua N">NH Chua</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoon, Ek" uniqKey="Yoon E">EK Yoon</name></author><author><name sortKey="Yang, Jh" uniqKey="Yang J">JH Yang</name></author><author><name sortKey="Lim, J" uniqKey="Lim J">J Lim</name></author><author><name sortKey="Kim, Sh" uniqKey="Kim S">SH Kim</name></author><author><name sortKey="Kim, Sk" uniqKey="Kim S">SK Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mallory, Ac" uniqKey="Mallory A">AC Mallory</name></author><author><name sortKey="Dugas, Dv" uniqKey="Dugas D">DV Dugas</name></author><author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name></author><author><name sortKey="Bartel, B" uniqKey="Bartel B">B Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Unver, T" uniqKey="Unver T">T Unver</name></author><author><name sortKey="Parmaks Z, I" uniqKey="Parmaks Z I">I Parmaksız</name></author><author><name sortKey="Dundar, E" uniqKey="Dundar E">E Dündar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kantar, M" uniqKey="Kantar M">M Kantar</name></author><author><name sortKey="Unver, T" uniqKey="Unver T">T Unver</name></author><author><name sortKey="Budak, H" uniqKey="Budak H">H Budak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fujii, H" uniqKey="Fujii H">H Fujii</name></author><author><name sortKey="Chiou, Tj" uniqKey="Chiou T">TJ Chiou</name></author><author><name sortKey="Lin, Si" uniqKey="Lin S">SI Lin</name></author><author><name sortKey="Aung, K" uniqKey="Aung K">K Aung</name></author><author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author><author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jung, Jh" uniqKey="Jung J">JH Jung</name></author><author><name sortKey="Seo, Yh" uniqKey="Seo Y">YH Seo</name></author><author><name sortKey="Seo, Pj" uniqKey="Seo P">PJ Seo</name></author><author><name sortKey="Reyes, Jl" uniqKey="Reyes J">JL Reyes</name></author><author><name sortKey="Yun, J" uniqKey="Yun J">J Yun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schommer, C" uniqKey="Schommer C">C Schommer</name></author><author><name sortKey="Palatnik, Jf" uniqKey="Palatnik J">JF Palatnik</name></author><author><name sortKey="Aggarwal, P" uniqKey="Aggarwal P">P Aggarwal</name></author><author><name sortKey="Chetelat, A" uniqKey="Chetelat A">A Chetelat</name></author><author><name sortKey="Cubas, P" uniqKey="Cubas P">P Cubas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jagadeeswaran, G" uniqKey="Jagadeeswaran G">G Jagadeeswaran</name></author><author><name sortKey="Saini, A" uniqKey="Saini A">A Saini</name></author><author><name sortKey="Sunkar, R" uniqKey="Sunkar R">R Sunkar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brodersen, P" uniqKey="Brodersen P">P Brodersen</name></author><author><name sortKey="Sakvarelidze Achard, L" uniqKey="Sakvarelidze Achard L">L Sakvarelidze-Achard</name></author><author><name sortKey="Bruun Rasmussen, M" uniqKey="Bruun Rasmussen M">M Bruun-Rasmussen</name></author><author><name sortKey="Dunoyer, P" uniqKey="Dunoyer P">P Dunoyer</name></author><author><name sortKey="Yamamoto, Yy" uniqKey="Yamamoto Y">YY Yamamoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, Zj" uniqKey="Yin Z">ZJ Yin</name></author><author><name sortKey="Li, Ch" uniqKey="Li C">CH Li</name></author><author><name sortKey="Han, Ml" uniqKey="Han M">ML Han</name></author><author><name sortKey="Shen, Ff" uniqKey="Shen F">FF Shen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Tz" uniqKey="Wang T">TZ Wang</name></author><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name></author><author><name sortKey="Zhao, Mg" uniqKey="Zhao M">MG Zhao</name></author><author><name sortKey="Tian, Qy" uniqKey="Tian Q">QY Tian</name></author><author><name sortKey="Zhang, Wh" uniqKey="Zhang W">WH Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Livak, Kj" uniqKey="Livak K">KJ Livak</name></author><author><name sortKey="Schmittgen, Td" uniqKey="Schmittgen T">TD Schmittgen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="German, Ma" uniqKey="German M">MA German</name></author><author><name sortKey="Luo, Sj" uniqKey="Luo S">SJ Luo</name></author><author><name sortKey="Schroth, G" uniqKey="Schroth G">G Schroth</name></author><author><name sortKey="Meyers, Bc" uniqKey="Meyers B">BC Meyers</name></author><author><name sortKey="Green, Pj" uniqKey="Green P">PJ Green</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Allen, E" uniqKey="Allen E">E Allen</name></author><author><name sortKey="Xie, Zx" uniqKey="Xie Z">ZX Xie</name></author><author><name sortKey="Gustafson, Am" uniqKey="Gustafson A">AM Gustafson</name></author><author><name sortKey="Carrington, Jc" uniqKey="Carrington J">JC Carrington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Mz" uniqKey="Li M">MZ Li</name></author><author><name sortKey="Xia, Yl" uniqKey="Xia Y">YL Xia</name></author><author><name sortKey="Gu, Yr" uniqKey="Gu Y">YR Gu</name></author><author><name sortKey="Zhang, K" uniqKey="Zhang K">K Zhang</name></author><author><name sortKey="Lang, Ql" uniqKey="Lang Q">QL Lang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Mao, Weihua" sort="Mao, Weihua" uniqKey="Mao W" first="Weihua" last="Mao">Weihua Mao</name></region><name sortKey="Li, Yadan" sort="Li, Yadan" uniqKey="Li Y" first="Yadan" last="Li">Yadan Li</name><name sortKey="Li, Zeyun" sort="Li, Zeyun" uniqKey="Li Z" first="Zeyun" last="Li">Zeyun Li</name><name sortKey="Mao, Weihua" sort="Mao, Weihua" uniqKey="Mao W" first="Weihua" last="Mao">Weihua Mao</name><name sortKey="Xia, Xiaojian" sort="Xia, Xiaojian" uniqKey="Xia X" first="Xiaojian" last="Xia">Xiaojian Xia</name><name sortKey="Yu, Jingquan" sort="Yu, Jingquan" uniqKey="Yu J" first="Jingquan" last="Yu">Jingquan Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001432 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001432 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3316546
|texte= A Combined Approach of High-Throughput Sequencing and Degradome Analysis Reveals Tissue Specific Expression of MicroRNAs and Their Targets in Cucumber
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22479356" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |